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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IP IH

Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrinmediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.
Catalog #	A1580-03C
Applications	Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:2,000
	Immunoprecipitation: 1-2ul/400ul lysates
	Immunohistochemistry: 1:100
	Immunocytochemistry: 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1, k
Clone No	10K104
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in Hepes, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.
Purity	Ascites
Immunogen	Recombinant human GSTfragment protein (1-347AA) purified from E.coli.
Specificity	Recognizes human Amphiphysin. Species crossreactivity: mouse, rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.