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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Amphiphysin (AMPH, AMPH1)

Anti -Amphiphysin (AMPH, AMPH1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IH IC
Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrinmediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.
Catalog #A1580-03D
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:2,000
Immunoprecipitation: 1-2ul/400ul lysates
Immunohistochemistry: 1:100
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Clone TypeMonoclonal
IsotypeIgG1k
Clone No10K105
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in Hepes, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.
PurityAscites
ImmunogenRecombinant human GST-fragment protein (320-696AA) purified from E.coli.
SpecificityRecognizes human Amphiphysin. Species crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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