Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Disease Markers » Anti -AMPH (AMPH1, amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen), HGNC:471) (CT)

Anti -AMPH (AMPH1, amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen),
HGNC:471) (CT)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Rabbit Serum B IP IH IC FC
Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).
Catalog #A1580-03F
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:20,000-1:100,000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:250-1:500
Flow Cytometry: 1:160
Immunoprecipitation: 1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E135
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySerum
ImmunogenA synthetic peptide corresponding to residues near C-terminus of human Amphipysin
SpecificityRecognizes human AMPH. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links