| Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3). |
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| Catalog # | A1580-03F |
| Applications | Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:20,000-1:100,000 |
| Immunohistochemistry: 1:100-1:250 |
| Immunocytochemistry: 1:250-1:500 |
| Flow Cytometry: 1:160 |
| Immunoprecipitation: 1:20 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 9E135 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. |
| Purity | Serum |
| Immunogen | A synthetic peptide corresponding to residues near C-terminus of human Amphipysin |
| Specificity | Recognizes human AMPH. Species Crossreactivity: mouse and rat |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
