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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B

AMPH is a member of the Amphiphysin family, BAR superfamily of proteins. Although its calculated MW is only 78kD, it runs anomalously in SDS-PAGE at 125-128kD. It is expressed in neurons, Sertoli cells, possibly in anterior pituitary endocrine cells and apparently cardiac muscle. It may form heterodimers with AMPH2, and in this configuration, promotes clathrin-associated endocytic vesicle formation. Furthermore, and perhaps related, AMPH also interacts with N-WASP, initiating actin polymerization. Human AMPH is 695aa in length. It contains an N-terminal BAR domain aa24-240 that binds lipids, a central region that binds AP2 aa322-340 and clathrin aa347-386, and a C-terminal SH3 domain aa622-695 that binds proline-rich ligands. There is one known 108kD splice variant that shows a deletion of aa425-466. Over aa204-300, human AMPH shares 98% aa identity with mouse AMPH.
Catalog #	A1580-03J
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 0.5ug/ml
	Optimal dilutions to be determined by the researcher.
	Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2B
Clone No	12K8
Host	Mouse
Source	Human
Concentration	~0.5mg/ml after reconstitution
Form	Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Recombinant corresponding to aa204-300 from human AMPH expressed in E.coli.
Specificity	Recognizes human Amphiphysin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.