Anti -Amphiregulin (Schwannoma-derived Growth Factor, Amphiregulin precursor, AR,
Colorectum cell-derived growth factor, CRDGF, MGC13647, SDGF) (BSA & Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E IP |
|
| Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84aa residue glycosylated polypeptide growth regulator. It is generated by proteolytic processing of a 252aa transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes are capable of binding to the extracellular domain of EGFR. They include EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein is localized to the epithelium of the colon, stomach, pancreas, breast and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas. | | | Catalog # | A1585X | | Applications | Suitable for use in ELISA and Immunoprecipitation. Other applications not tested | | Recommended Dilution | Immunoprecipitation (Native verified): 10ug/mg protein lysate. Use Protein A. | | Optimal dilutions to be determined by the researcher. | | Positive Control | LS174T cells | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, before the addition of glycerol to 40%. No stabilizing protein and no preservatives added. Also available with BSA and sodium azide. See Cat# A1585. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa8-26 (VKPPQNKTESENTSDKPKR) from the secreted amphiregulin of human origin. MW: 18.5-22.5kD | | Cellular Localization | Cytoplasmic and/or nuclear | | Specificity | Recognizes human Amphiregulin. Species Crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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