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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -Amphiregulin (Schwannoma-derived Growth Factor, Amphiregulin Precursor, AR, Colorectum Cell-derived Growth Factor, CRDGF, MGC13647, SDGF)

Anti -Amphiregulin (Schwannoma-derived Growth Factor, Amphiregulin Precursor, AR,
Colorectum Cell-derived Growth Factor, CRDGF, MGC13647, SDGF)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
The mature secreted form Amphiregulin (AR) is an 84-amino acid residue glycosylated polypeptide which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR expression is induced by phorbol ester, estrogen, androgen and other ligands of EGFR. In vitro, AR functions as an autocrine growth stimulator to derive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells and keratinocytes. AR is important in development of human placenta and murine mammary gland. AR is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.
Catalog #A1590
ApplicationsSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (native verified): 10ug/mg protein lysate. Use Protein A.
Western Blot: 5ug/ml for 2hrs at RT.
Optimal dilutions to be determined by the researcher.
Positive ControlLS174T and HT29 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also supplied without BSA and sodium azide. See A1590X.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic 19-mer peptide, corresponding to aa26-44 from secreted Amphiregulin of human origin. Cellular Localization
SpecificityRecognizes human Amphiregulin. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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