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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -Amphiregulin (Schwannoma-derived Growth Factor, Amphiregulin precursor, AR, Colorectum cell-derived growth factor, CRDGF, MGC13647, SDGF) (BSA & Azide Free)

Anti -Amphiregulin (Schwannoma-derived Growth Factor, Amphiregulin precursor, AR,
Colorectum cell-derived growth factor, CRDGF, MGC13647, SDGF) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Directed against the region of AR which binds to heparin and is highly specific to AR. It shows no crossreaction with EGF, TGFa, or HB-EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds.
AR expression is induced by phorbol ester, estrogen, androgen, and other ligands of EGFR. In vitro, AR functions as an autocrine growth stimulator to drive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. AR is important in development of human placenta and murine mammary gland. AR is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.
Catalog #A1590X
ApplicationsSuitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionsImmunoprecipitation (Native verified): 10ug/mg protein lysate. Use Protein A.
Western Blot: 5ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Positive ControlLS174T cells; HT29 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See A1590.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic 19-mer peptide corresponding to aa26-44 of human mature secreted amphiregulin. Cellular Localization:
Cytoplasmic and/or nuclear. MW of Antigen18.5-22.5kD
SpecificityRecognizes human amphiregulin. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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