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You are here:Home » Molecular Biology » MB-Enzymes, Glycosidase » Amylase, alpha, Human Pancreas

Amylase, alpha, Human Pancreas


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Alpha amylase is able to catalyze the hydrolysis of starch (amylose and amylopectin) to a significant extent resulting in the release of maltose. The enzyme is found in nearly all plants, animals and microorganisms. The enzymes from various sources exhibit marked differences in physical, chemical and catalytic properties. Bacterial amylases and those from porcine pancreas and human saliva have been extensively studied. Alpha amylase is widely used in the food industry as a clarifying agent. Determination of serum alpha amylase activity, for the diagnosis of acute pancreatitis, is a widely used procedure in clinical medicine (Brooks, F., New Eng. J. Med., 286, 300, 1972). Additionally, it has been reported that elevated alpha amylase activity in serum also occurs in mumps, renal disease and abdominal disorders such as cholecystitis (Salt, W. and Schenkder, S., Medicine, 55, 269, 1978).
Catalog #A2275-09
Protein 1mg protein/ml (A280, E0.1%=2.33)
Activity 400U/ml (Siemens Dimension® Clinical Chemistry System)
Specific Activity:
100U/mg protein
Unit DefinitionOne unit will catalyze the hydrolysis of one micromole of 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C.
ContaminantsGOT/AST: 0.01%
ALP: 0.01%
Lipase: 0.1%
Protease: 0.01%
Storage and StabilityAliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number9000-90-2
SourceHuman Pancreas
Concentration~2mg/ml Protein
FormSupplied as a liquid in 100mM Tris-HCl, 150mM sodium chloride, 1mM calcium chloride, 1mM benzamidine, 1mM pefabloc, pH 7.5. No preservatives added.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesa-Amylase, 1,4-a-D-glucan glucanohydrolase, glycogenase

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