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Amylase, alpha, Enzyme Assay Kit, BioAssay™
Amylase, alpha, Enzyme Assay Kit, BioAssay™
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| Catalog # | A2275-19 | | Description | Amylase belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on á-1,4- glycosidic bonds. The á-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, á-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure. Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. Enzyme a-amylase assay method involves two steps: (1). á-amylase in the sample hydrolyzes starch and the product is rapidly converted to glucose by á- glucosidase and hydrogen peroxide by glucose oxidase; (2). hydrogen peroxide concentration is determined with a colorimetric reagent. | | Applications | Direct assays of á-amylase activity in blood, saliva, urine, grains and other agricultural samples. | | Key Features | Sensitive and accurate. Linear detection range 0.3 to 50 U/L á- amylase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, incubation for 15 min, followed by the detection reagent and a 20-min incubation and reading the optical density at 585 nm. | | Kit Contents Assay Buffer (pH 7.0) | 20ml Substrate: 120ml Detection Reagent: 20ml Enzyme A: 220ml Glucose Standard: 1ml Enzyme B: 2 x 120ml Storage conditions. Kit is shipped on dry ice. Store Substrate and Detection Reagent at 4°C and others at -20°C. Shelf life of at least 6 months. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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