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You are here:Home » Molecular Biology » MB-Enzymes, Glycosidase » Amylase, alpha, Porcine Pancreas

Amylase, alpha, Porcine Pancreas

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Specifications

Alpha Amylase acts upon large polysaccharides at the alpha-1,4-glucoside bonds. The hydrolytic products have alpha configuration. The activity is present in all living organisms, however the enzymes vary remarkably even from tissue to tissue within a single species.
Catalog #A2275-21
SourceAmylase, alpha, from porcine pancreas.
Activity~230Units/mg powder @ 37°C
Protein~0.4mg Protein/mg powder
Specific Activity~480Units/mg
Unit DefinitionOne unit will catalyze the hydrolysis of one micromole of 2-chloro-4-mitrophenyl-alpha-D-maltrtrioside to yield 2-chloro-4-nitrophenol per minute at 37°C.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile tris saline buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number9000-90-2
SourcePorcine pancreas
PurityPurified. Contaminants
ConcentrationNot determined
FormSupplied as a lyophilized powder from 150mM sodium chloride, 3mM calcium chloride. Reconstitute with Tris saline buffer.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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