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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IH IF

Catalog #	A2275-54
Applications	Suitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry: (frozen)- 1:100 Immunohistochemistry:(paraffin)- 1:100.
	Does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
	Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents	I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
	I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Hybridoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H529
Host	Mouse
Source	Human
Concentration	Not determined.
Form	Supplied as a liquid with 0.09% sodium azide.
Purity	Tissue culture supernatant.
Immunogen	CATQRLANFLV coupled to tuberculin.
Specificity	Recognizes the human islet amyloid polypeptide, Amylin. The antibody is of high affinity and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.