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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified IH

Amylin is a pancreatic islet peptide with a role in the maintenance of glucose homoeostasis. It is
predominantly found in the beta cells of the pancreas and to a lesser extent in the gastrointestinal tract and nervous system. This antibody may prove of value in studies to determine the level of staining for amylin in normal and diabetic pancreas and pancreatic carcinoma.
Catalog #	A2275-54A
Applications	Suitable for use in Immunohistochemistry (formalin-fixed, paraffin-embedded tissues). Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:25 to 1:50 for 30 min. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilution to be determined by the researcher.
Positive Control	Pancreas.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide before the addition of glycerol to 40%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide derived from N-terminus of human amylin peptide. Cellular Localization
Specificity	Recognizes human Amylin peptide
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.