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You are here:Home » Antibodies » Abs to Amyloid » Anti -Amyloid Beta (NT) (Amyloid A4)

Anti -Amyloid Beta (NT) (Amyloid A4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IC
Amyloid b (Ab) accumulates in the senile plaques of the brain during Alzheimer’s disease and during certain classes of cerebrovascular angiopathy. Ab generation is initiated by proteolytic cleavage of the Amyloid Precursor Protein (APP) by b-secretase (BACE) at a site which corresponds to the Ab N terminus. A second proteolytic cleavage by g-secretase at a site corresponding to the Ab C terminus releases the Ab peptide. The insoluble Ab peptide forms aggregates in the vessels and senile plaques of Alzheimer’s disease brains.
Catalog #A2275-59L
ApplicationsSuitable for use in ELISA, Western Blot, and Immunocytochemistry. Other applications not tested.
Recommended DilutionsELISA: 0.05ug/ml
Western Blot: 1ug/ml for AP/BCIP/NBT; 0.1ug/ml for HRP/ECL detection
Immunocytochemistry: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9L27
HostMouse
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, PEG, sucrose, 0.1% sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by thiophilic adsorption and size exclusion chromatography.
ImmunogenSynthetic peptide corresponding to N-terminal Amyloid beta, conjugated to KLH
SpecificityRecognizes the free N-terminus of the beta A-4 polypeptide with high preference. Shows only minor crossreactivity with Amyloid Precursor Protein (APP).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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