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You are here:Home » Antibodies » Abs to Amyloid » Anti - -Amyloid, Oligomer A11

Anti - -Amyloid, Oligomer A11

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP IH IF
Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. In disease state oligomerization, extensive amyloid oligomerization creates plaques in neural tissue that correlates with Alzheimer’s symptomology.
Catalog #A2275-68A
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: Direct
Western Blot: 1:500 detected Amyloid Oligomer on 10ug of mouse brain lysates.
Immunoprecipitation: 1:1000. Suggested cell lysis buffer is RIPA. Suggested capture agent is magnetic beads (Dynabeads). Known co-precipitatiing polypeptide: Amyloid beta, alpha synuclein oligomers.
Immunofluorescence: 1:200 diluted 1:1 in a mixture of blocking solution and PBS (refer to protocol)
Immunohistochemistry: 1:1000-1:10,000
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid. No preservative added.
PuritySerum
SpecificityRecognizes human b-Amyloid, oligomer A11. Recognizes all types of amyloid oligomers. Appears to recognize a peptide backbone epitope that is common to amyloid oligomers, but is not found in native proteins, amyloidogenic monomer or mature amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation.
Species CrossreactivityMouse, rat, eukaryote.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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