| Accumulation of the amyloid-b peptide (Ab) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The b-amyloid protein precursor (APP) is cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generatethe 4kD amyloid-b peptide(Ab), which is deposited in the brains of all suffers of Alzheimer’s disease. |
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| Catalog # | A2275-69C |
| Application(s) | |
| Western Blot for detection of APP and Ab peptide. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:500-1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Murine brain tissue lysate. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
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| Host | Rabbit |
| Source | Human |
| Concentration | ~0.5mg/ml |
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| Form | Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Peptide sequence corresponding to amino acids 653 to 662 of human amyloid A4 protein precursor (APP). Identical to that of rabbit, porcine, bovine, guinea pig and chicken. |
| Specificity | Recognizes Amyloid Precursor Protein. Species Crossreactivity: Human, Rat and Mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | A4, AAA, ABETA, ABPP, AD1, Alzheimer disease amyloid protein, amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease), Amyloid beta A4 protein precursor, APPI, Cerebral vascular amyloid peptide, CTFgamma, CVAP, PN2, PN-II, PreA4, Prote |
