| Catalog # | A2275-69L |
| Sensitivity | <0.4ng/ml The analytical sensitivity of this assay is <0.4ng/ml of Human APP. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 30 times. |
| Specificity | The Human APP BioAssay™ ELISA kit is specific for measurement of total APP protein. The following proteins were tested at 100ng/ml and found to have no crossreactivity: Tau, a-synuclein, b-synuclein, Ab1-40 and Ab1-42. |
| Range | 0.78-50ng/ml |
| Incubation Time | 4 hours |
| Sample Size | 10ul (diluted to a total volume of 100ul) |
| Sample Type | Cell lysates and cerebral spinal fluids |
| Specifications | Simple: Pre-coated strip-well plates. |
| Liquid stable chromogen and stop reagents. |
| Economical: One 96-well plate, plus reagents for 96 determinations. |
| Reagents are stable for multiple runs. |
| Precision | Intra-assay: <8% |
| Inter-assay: <9% |
| Kit Components | *A2275-69L1: Standard, 2x1 vial |
| Supplied as a lyophilized powder. Reconstitute with 1ml of A2275-69L2: Standard Diluent Buffer. |
| A2275-69L2: Standard Diluent Buffer,1x25ml |
| Supplied as a liquid containing 8mM sodium azide. |
| A2275-69L3: Microtiter Plate, 1x96 wells |
| A2275-69L4: anti-APP (Biotin), 1x11ml |
| A2275-69L5: Streptavidin (HRP) (100X), 1x125ul |
| Supplied as a liquid containing 1.7mM thymol. |
| A2275-69L6: Streptavidin (HRP) Diluent, 1x25ml |
| Supplied as a liquid containing 1.7mM thymol and 0.05% Proclin 300. |
| A2275-69L7: Wash Buffer (25X), 1x100ml |
| A2275-69L8: TMB, 1x25ml |
| A2275-69L9: Stop Solution, 1x25ml |
| Storage and Stability | Store *A2275-69L1: Standard powder at 4°C, liquid at -70°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
