| Amyloid Precursor Protein IS a 130kD member of the APP family, expressed in adult brain. It is a cell surface kinesin I membrane receptor which regulates the axonal transport of beta-secretase and presenilin 1.In Alzheimer's disease processing of APP by beta-secretase gives rise to amyloidogenic 42 AA beta amyloid protein that is found in senile plaques and vessels. APP binds components of the extracellular matrix such as heparin and collagen I and IV, regulating neurite growth and is also involved in neuronal adhesion, cell mobility and axonogenesis. Through it’s binding to APBB1/Tip60, APP plays a role in the regulation of transcription. APP also inhibits Notch signaling through it’s interaction with NUMB. |
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| Catalog # | A2275-69V |
| Applications | Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:1500 |
| Immunohistochemistry (Frozen): 1:5,000 |
| Immunohistochemistry (Paraffin): 1:100 |
| Western Blot: 1:1000. Detects a band of ~130kD in neuronal cell lysates. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid, 0.09% sodium azide |
| Purity | Serum |
| Immunogen | Synthetic peptide corresponding to the aa44-63 of human amyloid precursor protein (Swiss Prot #P05067) |
| Specificity | Recognizes human Amyloid Precursor Protein. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | A4, AAA, ABETA, ABPP, AD1, Alzheimer Disease Amyloid Protein, Amyloid beta (A4) Precursor Protein (Peptidase Nexin-II, Alzheimer Disease), Amyloid beta A4 Protein Precursor, APPI, Cerebral Vascular Amyloid Peptide, CTFgamma, CVAP, PN2, PN-II, PreA4, Prote |
