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You are here:Home » Antibodies » Abs to Amyloid » Anti - -Amyloid Precursor Protein (APP), phosphorylated (Thr668) (BSA, Azide, Glycerol Free)

Anti - -Amyloid Precursor Protein (APP), phosphorylated (Thr668) (BSA, Azide, Glycerol
Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Amyloid beta (A4) precursor protein (APP) is a 100-140kD transmembrane glycoprotein existing in several different isoforms. The amino acid sequence of APP contains the amyloid domain (Abeta), which can be released by a two-step proteolytic cleavage. The extracellular deposition and accumulation of the released Abeta fragments form the main components of amyloid plaque in Alzheimer’s disease. APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion pathway of this protein. The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle (G2/M-phase) dependent. The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation.
Catalog #A2275-70X
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration0.5mg/ml
FormSupplied as a liquid in PBS. No stabilizing proteins added. No preservative added. Glycerol free. Also available with BSA. See A2275-70.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr668 of human APP695.
SpecificityDetects different isoforms of amyloid beta (A4) precursor protein only when phosphorylated at Thr668 (position corresponds to APP695 isoform). Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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