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You are here:Home » Antibodies » Abs to Amyloid » Anti -Amyloid Precursor Protein, CT (APP, Amyloid beta (A4) Precursor Protein)

Anti -Amyloid Precursor Protein, CT (APP, Amyloid beta (A4) Precursor Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IH IC
Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients.
Catalog #A2275-71B
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 1:100-1:400
Immunocytochemistry on NTera2 and COS cell lines: 1:100-1:400
Western Blot: 1:500-1:2000
Immunoprecipitation: 1:100-1:400.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PuritySerum
ImmunogenNine amino acid peptide from the C-terminus of APP
SpecificityAmyloid Precursor Protein (APP), C-terminal. May react with APLP1 and APLP2. Species Crossreactivity: Human and monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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