Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Antibodies-Amyloid » Anti -Amyloid Precursor Protein, CT (APP, Amyloid beta (A4) Precursor Protein)

Anti -Amyloid Precursor Protein, CT (APP, Amyloid beta (A4) Precursor Protein)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IH IC
Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients.
Catalog #A2275-71B
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: 1:100
Western Blot: 1:500-1:2000. The low abundance of full length APP in untreated cells means that poor response is seen with Western Blot of whole cell lysates. We recommend that membrane fractions be prepared to increase the loading of APP as well as remove potential interferences from soluable proteins. NTera2, Cos7 and Hela cell membrane preparations are positive by Western Blots.
Immunoprecipitation: 1:100-1:400
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot Determined
FormSupplied as a liquid, 0.02% sodium azide.
PuritySerum
ImmunogenNine amino acid peptide from the C-terminus of APP
SpecificityRecognizes Amyloid Precursor Protein (APP), C-terminal. May react with APLP1 and APLP2. Species Crossreactivity: human and monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links