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Anti -Amyloid Precursor Protein, CT (APP, Amyloid beta (A4) Precursor Protein)
Anti -Amyloid Precursor Protein, CT (APP, Amyloid beta (A4) Precursor Protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IP IH IC |
|
| Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients. | | | Catalog # | A2275-71B | | Applications | Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested. | | Recommended Dilution | Immunohistochemistry: 1:100-1:400 | | Immunocytochemistry on NTera2 and COS cell lines: 1:100-1:400 | | Western Blot: 1:500-1:2000 | | Immunoprecipitation: 1:100-1:400. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in PBS, 0.02% sodium azide. | | Purity | Serum | | Immunogen | Nine amino acid peptide from the C-terminus of APP | | Specificity | Amyloid Precursor Protein (APP), C-terminal. May react with APLP1 and APLP2. Species Crossreactivity: Human and monkey. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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