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You are here:Home » Antibodies » Abs to Amyloid » Anti -Amyloid Precursor Protein, Kpi Domain (APP, Amyloid beta (A4) Precursor Protein)

Anti -Amyloid Precursor Protein, Kpi Domain (APP, Amyloid beta (A4) Precursor Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IH IC
Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients.
Catalog #A2275-71E
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern blot: 1:250–1:1000
Immunoprecipitation: Requires reduction and alkylation of disulfide bonds. Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulfide bonds.
Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulfide bonds or microwaving.
To reduce: Incubate the slide or Blot in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at RT. Wash twice with TBS (PBS), block and process as usual.
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4, 0.01% sodium azide.
PuritySerum
ImmunogenAPP
SpecificityRecognizes human Amyloid Precursor Protein (APP), KPI Domain. Species crossreactivity: mouse, monkey and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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