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Anti -Amyloid Precursor Protein, Kpi Domain (APP, Amyloid beta (A4) Precursor Protein)
Anti -Amyloid Precursor Protein, Kpi Domain (APP, Amyloid beta (A4) Precursor Protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IP IH IC |
|
| Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients. | | | Catalog # | A2275-71E | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilution | Western blot: 1:250–1:1000 | | Immunoprecipitation: Requires reduction and alkylation of disulfide bonds. Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulfide bonds. | | Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulfide bonds or microwaving. | | To reduce: Incubate the slide or Blot in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at RT. Wash twice with TBS (PBS), block and process as usual. | | Optimal dilutions to be determined by researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in PBS, pH 7.4, 0.01% sodium azide. | | Purity | Serum | | Immunogen | APP | | Specificity | Recognizes human Amyloid Precursor Protein (APP), KPI Domain. Species crossreactivity: mouse, monkey and bovine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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