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You are here:Home » Antibodies » Abs to Amyloid » Anti -Amyloid Precursor Protein, Secretory (sAPP)

Anti -Amyloid Precursor Protein, Secretory (sAPP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment
(sbAPP) with Mr 100kD and a C-terminal fragment (C99) with Mr 12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients.
Catalog #A2275-71H
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 10ug/ml
ELISA: 1ug/ml
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No2Q18
HostMouse
SourceHuman
Concentration~3mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenAmyloid Precursor Protein (APP) secreted by the EJ-1 bladder carcinoma cell line.
SpecificityRecognizes secretory amyloid precursor protein (sAPP). Does not recognize Amyloid-Beta protein 1-40.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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