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You are here:Home » Antibodies » Abs to Amyloid » Anti -Amyloid Precursor Protein, Secretory (sAPP)

Anti -Amyloid Precursor Protein, Secretory (sAPP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr 100kD and a C-terminal fragment (C99) with Mr 12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients.
Catalog #A2275-71H1
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1ug/ml
Western Blot: 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No9G303
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
PurityPurified by Protein A affinity chromatography
ImmunogenAmyloid protein precursor (APP) secreted by the EJ-1 bladder carcinoma cell line.
SpecificityRecognizes human secretory amyloid protein precursor (sAPP). Does not recognize bAP1-40.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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