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You are here:Home » Antibodies » Abs to Apoptosis » Anti -Amyloid Precursor Protein, Kpi Domain (APP, Amyloid beta (A4) Precursor Protein)

Anti -Amyloid Precursor Protein, Kpi Domain (APP, Amyloid beta (A4) Precursor Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IH
Only the APP-751 and APP-770 isoforms contain a KPI domain, it being absent from the APP-695 isoform that is most abundant in neuronal tissue. However, there is evidence that suggests KPI contain isoforms may be important in the pathogenesis of Alzheimer disease.
Catalog #A2275-71P
ApplicationsSuitable for use in Immunohistochemistry, Immunoprecipitation, and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (paraffin): 1:400. Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. 0.05M Tris pH 7.0, followed by reduction and alkylation is recommended.
Immunoprecipitation: Neat, 5ul/ml after reduction and alkylation
Western Blot: 1:500-1:1000. Detects a band of approximately 115kD in NTera2/DI cell lysates.
Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents Sheep anti Rabbit IgG (Aff. Pur.)-HRP
Recommended Positive Control Tissue Alzheimers disease brain
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid containing 0.02% sodium azide.
PuritySerum.
ImmunogenSynthetic peptide composed of amino acids 292-312 from the KPI domain of human APP.
SpecificityRecognizes an epitope in the Kunitz protease inhibitor (KPI) domain of human amyloid precursor protein (APP). Species Crossreactivity: monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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