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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » -Amyloid, aa1-40, Human, BioAssay™ ELISA Kit

-Amyloid, aa1-40, Human, BioAssay™ ELISA Kit

Pricing

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Specifications

The United States Biological Human beta Amyloid 1-40 (Hu A beta40) ELISA kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for the NH2-terminus of Hu b-amyloid has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known Hu b-amyloid 40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-40 b-amyloid sequence. This COOH- terminal sequence is created upon cleavage of the analyzed precursor. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu A beta40 present in the original specimen.
Catalog #A2275-73M
Sensitivity<6pg/ml
Range7.8-500pg/ml
Kit Components*A2275-73M1: beta Amyloid 1-40 Standard, 1x1vial
A2275-73M2: Standard Diluent Buffer, 1x60ml
A2275-73M3: Microtiter Plate, 1x96 wells
A2275-73M4: beta Amyloid 1-40, Pab 1x6ml
A2275-73M5: IgG (HRP) (100X), 1x125ul
A2275-73M6: HRP Diluent, 1x25ml
A2275-73M7: Wash Buffer (25X), 1x100ml
A2275-73M8: TMB, 1x25ml
A2275-73M9: Stop Solution, 1x25ml
Storage and StabilityStore *A2275-73M1 powder at 4°C liquid at -70°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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