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You are here:Home » Antibodies » Abs to Amyloid » Anti - -Amyloid 1-40, 42

Anti - -Amyloid 1-40, 42

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 aa peptides (beta amyloid1-40, 1-42, and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140 aa a-synucleins is identical with non- Ab component (NACP) of AD. The 134 aa human b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine may cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms: two large (140 aa SYN-1 & 149 aa SYN-2) and a small form (SYN-3, 42 aa) are produced by alternative splicing.
Catalog #A2275-74M
Rabbit anti-human beta Amyloid antibodies was raised using synthetic b-amyloid conjugated to BSA. Full-length b-amyloid 1-40 control immunogenic peptide is also available to confirm specificity of antibodies (A2275-75G).
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS containing 0.05% sodium azide
PuritySerum
ImmunogenSynthetic b-amyloid 1-40 conjugated to BSA
SpecificityRecognizes human b-amyloid 1-40 and b-amyloid 1-42. The peptide sequence has high degree of homology with mouse b-amyloid 1-40 and b-amyloid 1-42. Weak crossreactivity is observed with b-amyloid 1-28 and b-amyloid 12-28. No crossreactivity is observed with CGRP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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