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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH IF

Amyloid beta (A4) precursor protein (APP) is a 100–140kD transmembrane glycoprotein existing in several different isoforms. The amino acid sequence of APP contains the amyloid domain (A-beta), which can be released by a two-step proteolytic cleavage. The extracellular deposition and accumulation of the released A-beta fragments form the main
components of amyloid plaque in Alzheimer’s disease. APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion pathway of this protein. The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle (G2/Mphase) dependent. The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation.
Catalog #	A2275-75B
Applications	Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Western Blot: 1:1000
	Immunofluorescence (Paraffin): 1:250
	Immunohistochemistry (Paraffin): 1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity	Purified by Protein A and peptide affinity chromatography.
Immunogen	Synthetic peptide corresponding to residues at the amino terminus of human beta-amyloid peptides (KLH).
Specificity	Recognizes several isoforms of human beta-amyloid peptide (Abeta) such as beta-amyloid 40, beta-amyloid 42 (regardless of phosphoralytation state) and others at ~5kD.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.