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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IH

The progressive deposition of insoluble amyloid protein plaques in specific areas of the brain and its blood vessels is associated with a number of neuropathologies and is an early invariant feature of Alzheimer’s disease. The major component of these protein plaques is a peptide of 39-42 amino acids derived from a group of 695-770 amino acid precursor proteins collectively referred to as the b-amyloid precursor protein (Beta-APP). b-APP is highly conserved in vertebrates; however, its normal function has not been well defined. In each of the b-APPs, the b-Amyloid peptide (b-AP) is an internal peptide that begins about 99 residues from the carboxyterminal end of the b-APP and extends from the extracellular domain into the middle of the hydrophobic, membrane spanning domain of the precursor protein. The precise mechanism whereby b-AP accumulates in brain remains poorly defined.
Catalog #	A2275-75C
Applications	Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 0.1-0.2ug/ml
	ELISA: 0.5-1ug/ml
	Immunohistochemistry (alcohol-fixed paraffin-embedded or frozen tissue sections): 10ug/ml. It is necessary to pretreat the tissue with trypsin digestion
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1, k
Clone No	6A5
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS pH 7.4, containing 0.05% sodium azide
Purity	Purified from mouse ascites fluid
Immunogen	A 28 amino acid synthetic peptide derived from the b-amyloid peptide.
Specificity	Reacts with the 4-5kD b-amyloid peptide (b AP). High concentrations of antibody will also react with A-a, B-a and gamma subunits of fibrinogen
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.