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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B

Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid1-40, 1-42 and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140aa a-synucleins is identical with non- Ab component (NACP) of AD. The 134aa human b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine may cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms: two large (140 aa SYN-1 & 149 aa SYN-2) and a small form (SYN-3, 42 aa) are produced by alternative splicing.
Catalog #	A2275-75K
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
	Western Blot: 1:1000-1:5000 (ECL).
	Optimal dilutions to be determined by the researcher.
Recommended Control Peptide	Full length b-amyloid (1-40aa and 1-42aa).
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H2
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a lyophilized powder, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Purity	Ascites
Immunogen	Human beta Amyloid 1-40 (KLH). Epitope: Localized to the N-terminus of beta amyloid (1-13 aa of 1-40/42/43)
Specificity	Recognizes human b-amyloid1-40/42. . Species Sequence Homology: mouse, rat and many other species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.