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You are here:Home » Antibodies » Abs to Amyloid » Anti - -Amyloid 1-40, 42

Anti - -Amyloid 1-40, 42

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid1-40, 1-42 and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140aa a-synucleins is identical with non- Ab component (NACP) of AD. The 134aa human b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine may cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms: two large (140 aa SYN-1 & 149 aa SYN-2) and a small form (SYN-3, 42 aa) are produced by alternative splicing.
Catalog #A2275-75K
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Western Blot: 1:1000-1:5000 (ECL)
Optimal dilutions to be determined by the researcher.
Recommended Control PeptideFull length b-amyloid (1-40aa and 1-42aa)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.05% sodium azide.
PurityAscites
ImmunogenHuman beta Amyloid 1-40, conjugated to KLH. Epitope: Localized to the N-terminus of beta amyloid (1-13aa of 1-40/42/43)
SpecificityRecognizes human b-amyloid1-40/42. Species Sequence Homology: mouse, rat and many other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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