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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IC

The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and
the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major
secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40
(Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).
Catalog #	A2275-75L
Applications
	Suitable for use in ELISA, Immunocytochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions	ELISA: 0.05ug/ml
	Western Blot: 1ug/ml for HRPO/ECL detection. Block with Casein/Tween 20 based blocking and blot incubation buffer.
	Immunocytochemistry: 0.1-1ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6D4
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 2X PBS, 0.1% sodium azide, PEG, sucrose.
Purity	Purified by size exclusion chromatography.
Immunogen	Synthetic peptide corresponding to aa1-40 and aa1-42 of C-terminal human b-amyloid, conjugated to KLH.
Specificity	Recognizes human C-terminal b-amyloid (aa1-40 and aa1-42). Crossreactivity: APP.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.