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You are here:Home » Antibodies » Abs to Amyloid » Anti - -Amyloid 1-42

Anti - -Amyloid 1-42

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 aa peptides (beta amyloid1-40, 1-42, and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140 aa a-synucleins is identical with non- Ab component (NACP) of AD. The 134 aa human b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine may cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms: two large (140 aa SYN-1 & 149 aa SYN-2) and a small form (SYN-3, 42 aa) are produced by alternative splicing.
Catalog #A2275-75S
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL.
ELISA: 1:10,000-1:100,000. Coat with 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide A2275-74P
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide consisting of 6aa of human beta 1-42 within to the C-terminal, conjugated to KLH.
SpecificityRecognizes b-amyloid 1-42. Sequence sequence homology: Mouse b-1-42. Demonstrates minimal crossreactivity with b-1-40.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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