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You are here:Home » Antibodies » Abs to Amyloid » Anti - -Amyloid Peptide

Anti - -Amyloid Peptide

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
The progressive deposition of insoluble amyloid protein plaques in speci c areas of the brain and its blood vessels is associated with a number of neuropathologies and is an early invariant feature of Alzheimer’s disease. The major component of these protein plaques is a peptide of 39-42 amino acids derived from a group of 695-770 amino acid precursor proteins collectively referred to as the b-amyloid precursor protein (Beta-APP). b-APP is highly conserved in vertebrates; however, its normal function has not been well de ned.
In each of the b-APPs, the b-Amyloid peptide (b-AP) is an internal peptide that begins about 99 residues from the carboxy-terminal end of the b-APP and extends from the extracellular domain into the middle of the hydrophobic, membrane spanning domain of the precursor protein. The precise mechanism whereby b-AP accumulates in brain remains poorly de ned.
Catalog #A2275-77A
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunoprecipitation: 5-10ug; For immunoprecipitation of b-Amyloid peptide from cultured cells, it is necessary to mix the culture medium with an equal volume of 2X RIPA buffer.
Immunohistochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 30 amino acid synthetic peptide derived from the full length (1-43 amino acid) b-Amyloid peptide.
SpecificitySpeci c for the 4-5kD human b-Amyloid peptide derived by cleavage from the b-Amyloid precursor protein. Reactivity has been con rmed by Western Blot using a synthetic b-Amyloid peptide (1-40), by immunohistochemistry on Alzheimer’s diseased human brain sections and by immunoprecipitation of 35S-methionine labeled b-Amyloid peptide secreted by transfected culture cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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