| The progressive deposition of insoluble amyloid protein plaques in speci c areas of the brain and its blood vessels is associated with a number of neuropathologies and is an early invariant feature of Alzheimer’s disease. The major component of these protein plaques is a peptide of 39-42 amino acids derived from a group of 695-770 amino acid precursor proteins collectively referred to as the b-amyloid precursor protein (Beta-APP). b-APP is highly conserved in vertebrates; however, its normal function has not been well de ned. |
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| In each of the b-APPs, the b-Amyloid peptide (b-AP) is an internal peptide that begins about 99 residues from the carboxy-terminal end of the b-APP and extends from the extracellular domain into the middle of the hydrophobic, membrane spanning domain of the precursor protein. The precise mechanism whereby b-AP accumulates in brain remains poorly de ned. |
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| Catalog # | A2275-77A |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 0.1-1ug/ml |
| Western Blot: 1-3ug/ml |
| Immunoprecipitation: 5-10ug; For immunoprecipitation of b-Amyloid peptide from cultured cells, it is necessary to mix the culture medium with an equal volume of 2X RIPA buffer. |
| Immunohistochemistry: 5ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A 30 amino acid synthetic peptide derived from the full length (1-43 amino acid) b-Amyloid peptide. |
| Specificity | Speci c for the 4-5kD human b-Amyloid peptide derived by cleavage from the b-Amyloid precursor protein. Reactivity has been con rmed by Western Blot using a synthetic b-Amyloid peptide (1-40), by immunohistochemistry on Alzheimer’s diseased human brain sections and by immunoprecipitation of 35S-methionine labeled b-Amyloid peptide secreted by transfected culture cells. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
