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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Catalog #	A2275-80B
Synthetic amyloid b-peptide (aa5-16)	RHDSGYEVHHQK-NH2 represents part of the larger amyloid b-peptide that precipitates as amyloid plaques in the brain of patients with Alzheimer's disease (1). The plaque-related peptides are 40-43 aminoacid residues in length (2).
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	ELISA: 1:20,000
	Optimal dilutions to be determined by the researcher.
Hybridoma	X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.
Culture Medium	Dulbecco’s modified Eagle’s medium with 10% fetal calf serum
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	6F24
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Amyloid b-peptide (5-16) absorbed onto aluminum hydroxide gel.
Specificity	Recognizes human b-Amyloid. The binding of this antibody to amyloid b-peptide depends on the presence of at least amino acid residues 5-9. Epitope specificity differs from those of clones 6F22, 6F23, and 6F25.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.