Anti -Amyloid Precursor Protein. Frameshift Mutant (APP, Amyloid beta (A4) Precursor
Protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Serum |
E B IH |
|
| Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients. In Alzheimer’s disease, this protein is broken down and releases the 12 amino acid beta-amyloid protein that is found in senile plaques and vessels. The amyloid precursor protein is currently investigationed to determine how this protein is broken down abnormally in Alzheimer’s disease to give rise to the beta-amyloid protein which is present in senile plaques and vessels. | | | Catalog # | A2275-81 | | Applications | Suitble for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | ELISA: 1:5000 | | Western Blot: 1:3000 | | Immunohistochemistry (Formalin fixed, paraffin-embedded sections): 1:300 | | Optimal dilutions to determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 0.1% sodium azide. | | Purity | Serum | | Immunogen | A synthetic peptide corresponding to aa339-348, RGRTSSKELA of human AAP. | | Specificity | Recognizes the amino acid sequence 339-348 of human amyloid precursor protein frameshift mutant. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | A4, AAA, ABETA, ABPP, AD1, Alzheimer disease amyloid protein, amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease), Amyloid beta A4 protein precursor, APPI, Cerebral vascular amyloid peptide, CTFgamma, CVAP, PN2, PN-II, PreA4, Prote |
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