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You are here:Home » Antibodies » Abs to Amyloid » Anti -Amyloid Precursor Protein, CT (APP, Amyloid beta (A4) Precursor Protein)

Anti -Amyloid Precursor Protein, CT (APP, Amyloid beta (A4) Precursor Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Serum E B
The APP is a large transmembrane protein which is constitutively expressed by many cell types. Within the Golgi apparatus and endosome, APP is oriented such that its N-terminus is within the lumen while the C-terminus extends into the cytoplasm. Cleavage of APP by b-secretase (BACE) generates the N-terminus of the b amyloid peptide within C99, the C-terminal fragment. Further cleavage by g-secretase yields the b amyloid peptide. b amyloid is released into the extracellular space.
Catalog #A2275-81B
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:25,000
Western Blot: 1:5,000
Optimal dilutions to be determined by the researcher.
Recommended Positive Control Neuronal cell lysate.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the ori0ginal vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.1% sodium azide.
PuritySerum
ImmunogenA synthetic peptide corresponding to aa sequence 681-695 (681GYENPTYKFFEQMQN695) of the C-terminus of human Amyloid Precursor Protein.
SpecificityRecognizes the C-terminus of the human Amyloid Precursor Protein (APP), a protein with Mr 100kD. The smaller APP C-terminal fragment b (CTFb; ~8kD) is rapidly degraded and not detected by Western blot analysis of brain cell lysates. May crossreact with Amyloid Precursor-Like Protein2 (APLP2). This antiserum can routinely be used without further purification.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesA4, AAA, ABETA, ABPP, AD1, Alzheimer disease amyloid protein, amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease), Amyloid beta A4 protein precursor, APPI, Cerebral vascular amyloid peptide, CTFgamma, CVAP, PN2, PN-II, PreA4, Prote


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