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You are here:Home » Antibodies » Abs to Amyloid » Anti -Amyloid Precursor Protein, NT (APP, Amyloid beta (A4) Precursor Protein)

Anti -Amyloid Precursor Protein, NT (APP, Amyloid beta (A4) Precursor Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Serum E B IH
Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients.
Catalog #A2275-86
In Alzheimer’s disease, this protein is broken down and releases the 12 amino acid beta-amyloid protein that is found in senile plaques and vessels. The amyloid precursor protein is currently investigationed to determine how this protein is broken down abnormally in Alzheimer’s disease to give rise to the beta-amyloid protein which is present in senile plaques and vessels.
ApplicationsSuitable for use in ELISA, Immunohistochemistry, and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:1,500 using immunizing peptide as sample
Immunohistochemistry (FFPE): 1:100
Immunohistochemistry (frozen): 1:5,000
Western Blot: 1:1,000
Optimal dilutions to be determined by the researcher.
Control Peptide A2275-86A1
Recommended Positive ControlNeuronal cell lysate, Alzheimer’s disease brain.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid (neat serum) containing 0.1% sodium azide
PuritySerum
ImmunogenSynthetic peptide (HMNVQNGKWDSDPSGTKTCI) corresponding to aa44-63 of human amyloid precursor protein
SpecificityRecognizes human amyloid precursor protein
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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