Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Amyloid » Anti - -Amyloid Precursor Protein

Anti - -Amyloid Precursor Protein

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Amyloid-beta precursor protein (APP) is the source of the amyloid-beta (Ab) peptide found in neuritic plaques of Alzheimer’s disease (AD) patients. APP is aglycosylated transmembrane protein with a long extracellular N-terminal domain, a short intracellular C-terminal domain, and an Abeta-segment of intermediate length shared between the intra- and extracellular regions. APP is cleaved by alpha-secretase immediately before or after reaching the cell surface, releasing the nonamyloidogenic secreted form of the N-terminal soluble APP fragment (sAPPa). APP molecules that are not cleaved by alpha-secretase are internalized, and subjected to beta-site cleavage by beta-secretase, leaving behind a membrane-bound C-terminal stub. This terminal stub is the substrate for gamma-secretase, which cleaves the molecule at gamma-site(s) to release the 40aa, 42aa, or 43aa -long Ab peptides.
Catalog #A2275-87F
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot (Mouse): 1-3ug/ml. Detects a band representing the full-length protein at ~ 100kD, and a band at ~ 10-12kD representing the C99 C-terminal cleavage fragment. A major non-specific band has been observed at ~50kD in mouse brain homogenates.
Immunoprecipitation (Mouse): 7ug/reaction
Immunohistochemistry (FFPE, Human): 1-2ug/ml. Digestion with trypsin prior to staining is required.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the C-terminal region of the human and mouse amyloid-beta precursor protein (APP695).
SpecificityRecognizes human Amyloid Precursor Protein. Reactivity has been confirmed with APLP2 (amyloid precursor-like protein 2) -/- knock-out and wildtype mouse brain homogenates and APP695 C99 (C-terminal fragment)-transfected HEK293 cell lysates by Western Blot. Negative control tests with APP -/- knock-out and APP/APLP2 double knock-out mice have confirmed the specificity of this antibody; neither of the target bands were identified in these test samples. Reactivity has also been confirmed with human Alzheimer’s brain tissue by immunohistochemistry. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links