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You are here:Home » Antibodies » Abs to Amyloid » Anti - -Amyloid Precursor Protein

Anti - -Amyloid Precursor Protein

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IF
Senile plaques and neurofibrillary tangles in brain are hallmarks of Alzheimerís disease. Mutations in the beta-amyloid precursor protein (beta-APP) are contributory factors to Familial Alzheimerís Disease. The primary constituent of senile plaques are deposits of the 4 kD amyloid beta-peptide, derived from amyloid beta-precursor proteins by internal proteolysis. The
major brain beta-APP isoforms (APP695, 751, 770) are integral membrane proteins with a single large N-terminal extracellular domain, a single transmembrane domain, and a small cytoplasmic C-terminal tail. In the early 1990s, it was discovered that beta-APP is a useful marker for axonal injury. beta-APP is carried by fast anterograde transport vesicles to distal sites in the axon with the beta-APP C-terminus residing in the cytoplasm and the N-terminus in the vesicle. Upon axonal injury, beta-APP will pool in areas of impaired transport. beta-APP has been used as a marker of axonal injury in many studies of different pathologies, including: head trauma, HTLV-I-associated myelopathy, multiple sclerosis, shaken baby syndrome, and encephalomyelitis among others.
Catalog #A2275-87J
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorscence and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionELISA: 0.1-1ug/ml
Western Blot: 0.2-2ug/ml
Immunofluoresence: 1-5ug/ml
Immunohistochemistry: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 22 amino acid synthetic peptide derived from the C-terminus of the human beta-amyloid precursor protein (beta-APP). This sequence is 100% conserved in human, monkey, mouse, rat, guinea pig, and chicken beta-APP proteins.
SpecificityThis antibody can be used to specifically detect the beta-amyloid precursor protein. The antibody reacts with full-length (APP695, 751, 770) and N-terminal truncated forms of beta-APP. The antibody can also be used to detect the C-terminal membrane-anchored fragment of beta-APP that remains after alpha- or beta-secretase cleavage. This antibody does not detect the beta-APP product N-terminal to the gamma-secretase cleavage site. To the best of our knowledge, reliable immunodetection of this fragment, with any antibody, has not been reported in the literature. Reactivity with this antibody has been confirmed for human, mouse, pig, and rat. Based on sequence homology (see above) reactivity with other species, including monkey, guinea pig, and chicken beta-APP is highly likely. Zymedís CT695 antibody has demonstrated superiority in detecting axonal damage.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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