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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Anaplastic Lymphoma Kinase (Nucleophosmin, p80, ALK, NPM, B23, NPM-ALK)

Anti -Anaplastic Lymphoma Kinase (Nucleophosmin, p80, ALK, NPM, B23, NPM-ALK)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
The ALK gene is carried by chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as nucleophosmin or B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre-ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM-ALK) resulting from t(2;5) translocation is a protein of 80kD with the N-terminal portion of NPM linked to the complete intracellular portion of ALK. ALK is found in many large cell lymphomas. Karpas 299 and SU-DHL-1 lymphoid cell lines strongly express NPM-ALK.
Catalog #A2277-01
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoprecipitation: 5-10ug/IP reaction
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlsT cell lines with the NPM-ALK translocation, including Karpas 299 and TS cells lysates.
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Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G6
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein derived from the human ALK protein.
SpecificityRecognizes the human ~80kD ALK protein. Crossreactivity with related proteins has not been observed.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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