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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH IF Gel

The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormone-responsiveness.
Catalog #	A2281
Applications	Suitable for use in Gel Supershift, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Gel Supershift: Order Ab at 1mg/ml. See cat.# A2281X.
	Immunoprecipitation (Denatured): 2ug antibody/mg protein lysate (Use Protein G).
	Western Blot: 1ug/ml for 2hrs at RT.
	Immunohistochemistry (Formalin/paraffin): 1:50 for 30 min. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Positive Control	LnCap cells or prostate carcinoma.
Source	Ascites
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.15
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide before the addition of glycerol to 40%. Also available without BSA and azide. See A2281X.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Synthetic peptide corresponding to aa299-315
(STEDTAEYSPFKGGYTK) of human Androgen Receptor.
Cellular Localization	Nuclear. MW of antigen: 110kD
Specificity	Recognizes human androgen receptor at 110kD. Species Crossreactivity: Canine. Does not react with mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.