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You are here:Home » Antibodies » Abs to Receptors » Anti -Androgen Receptor

Anti -Androgen Receptor

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IF
The expression of androgen receptor (AR) is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormoneresponsiveness.
Catalog #A2281-01
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Denatured): 10ug/mg protein lysate
(Use Protein A).
Western Blot: 5ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 30 min at RT. Note: Requires boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlLnCap cells or prostate carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See A2281-01X
PurityPurified by Protein A affinity chromatography.
ImmunogenA synthetic peptide derived from N-terminus of rat AR. Cellular Localization
SpecificityRecognizes rat Androgen Receptor at 110kD. Species Crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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