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You are here:Home » Antibodies » Abs to Receptors » Anti -Androgen Receptor (AR, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, androgen receptor (dihydrotestosterone receptor testicular feminization spinal and bulbar muscular atrophy Kennedy disease), dihydrotestosterone receptor)

Anti -Androgen Receptor (AR, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, androgen
receptor (dihydrotestosterone receptor testicular feminization spinal and bulbar muscular
atrophy Kennedy disease), dihydrotestosterone receptor)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormone-responsiveness.
Catalog #A2281-01B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA:1:64000
Western Blot: 0.3-2ug/ml. ~95kD band observed in Human Brain and Human Heart lysates (calculated MW of 99kD). Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See A2281-01B-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenEVQLGLGRVYPRPPSC, N Terminus
SpecificityRecognizes Human Androgen Receptor. Species Sequence Homology: Mouse, Rat, and Chimpanzee.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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