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Anti -Androgen Receptor (Dihydrotestosterone Receptor, Nuclear Receptor Subfamily 3 Group C Member 4, AR, DHTR, NR3C4)
Anti -Androgen Receptor (Dihydrotestosterone Receptor, Nuclear Receptor Subfamily 3 Group C Member 4, AR, DHTR, NR3C4)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH FC |
|
| Catalog # | A2281-02X | | The expression of AR (MW | 70kD) is reportedly inversely correlated with histologic grade, i.e., well-differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormone-responsiveness. | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Gel Supershif Assay and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 2.5-5ug/ml for 2hrs at RT | | Immunoprecipitation: 2ug/mg protein lysate | | Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT | | Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min | | Optimal dilutions to be determined by the researcher. | | Positive Control | LnCap cell prostrate carcinoma | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Clone No | 2D52 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Synthetic peptide corresponding to a protein sequence from human AR. | | Specificity | Recognizes human Androgen Receptor. Does not react with mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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