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You are here:Home » Antibodies » Abs to Receptors » Anti -Androgen Receptor (Dihydrotestosterone Receptor, Nuclear Receptor Subfamily 3 Group C Member 4, AR, DHTR, NR3C4)

Anti -Androgen Receptor (Dihydrotestosterone Receptor, Nuclear Receptor Subfamily 3 Group
C Member 4, AR, DHTR, NR3C4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
Catalog #A2281-02X
The expression of AR (MW 70kD) is reportedly inversely correlated with histologic grade, i.e., well-differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormone-responsiveness.
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Gel Supershif Assay and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2.5-5ug/ml for 2hrs at RT
Immunoprecipitation: 2ug/mg protein lysate
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlLnCap cell prostrate carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No2D52
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to a protein sequence from human AR.
SpecificityRecognizes human Androgen Receptor. Does not react with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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