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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum B IH IC

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgen Receptors (AR) have been found in a variety of tissues, including reproductive organs, central nervous system and skeletal muscle.
Catalog #	A2281-05
Application(s)
	Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry By Western Blot, this antibody detects an ~110kD protein representing AR from rat brain homogenate. Faint bands at 70 and 75kD are also detected in both immune and pre-immune sera. Immunohistochemical staining of AR in rat brain results in intense nuclear staining. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 4ug/ml
	Western Blot: 4ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS with 0.05% sodium azide.
Purity	Whole antiserum
Immunogen	Synthetic peptide: M(1)EVQLGLGRVYPRPPSKTYRG(21)C. Immunizing peptide corresponds to the N-terminal 21 amino acids of human AR. This sequence is completely conserved between human, mouse and rat AR.
Specificity	Detects Androgen Receptor. Does not detect estrogen, progesterone, or glucocorticoid receptors. Species Crossreactivity: Mouse, Non Human Primates and Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.