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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

Angiocidin (also S5a, RPN10a and Mcb1) is a 41kD (predicted) member of the proteosome subunit S5A family of proteins. It runs anomalously in SDS-page at 55kD. Intracellularly, Angiocidin is a component of the 26S proteosome that degrades ubiquitinated proteins. Extracellularly, it inhibits angiogenesis by binding to collagen and beta 1 integrins. Mouse Angiocidin is 376aa in length. It contains a vWF-A domain (aa5-188), a beta 1 interacting site (aa86-105) and two ubiquitin-interacting motifs (aa211-230 and 282-301). There are four splice variants. Three show a GlyGluArg insertion after Glu254, with two of these showing an additional three aa substitution for aa255-376, and a 45aa substitution for aa321-376, respectfully. A fourth shows a 51aa substitution for aa299-376. Over aa1-254, mouse Angiocidin shares > 99% aa identity with rat and human Angiocidin.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Catalog #	A2292-23
Recommended Dilution	ELISA: 0.5-1.0ug/ml
	Western Blot: 1.0ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Mouse
Concentration	~0.2mg/ml
Form	Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Recombinant mouse Angiocidin isoform A aa2-254; O35226 (E. coli).
Specificity	Recognizes mouse Angiocidin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.