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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B N

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis. Angiogenin is a single polypeptide chain of 123aa (mol wt ~14kD) secreted by tumor cells. It is a potent inhibitor of neovascularization. It specifically binds to endothelial cells and elicits second messenger systems. It has ribonucleolytic activity and is 33% identical to pancreatic RNAse A.
Catalog #	A2292-53B
Applications	Suitable for use in Western Blot, ELISA and Neutralization. Other applications not tested.
Recommended Dilution	Western Blot: 1-5ug/ml using ECL.
	ELISA: 0.5-2.0ug antibody and 10-50ng of control antigen/well.
	Neutralization: 100-200ug IgG neutralizes 50% activity of 1ug of Angiogenin. Neutralizes biological activity of recombinant human Angiogenin as measured by measurement of ribonucleolytic activity using tRNA.
	Optimal dilution determined by the researcher.
Recommended Control	A2292-53C: Angiogenin, Recombinant, Human, Western Blot Control
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 20mM Tris, pH 7.4, 0.1% BSA.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Human Angiogenin protein expressed in E. coli.
Specificity	Recognizes human Angiogenin at ~14kD.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.