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You are here:Home » Antibodies » Abs to Angiopoietin » Anti -Angiopoietin 1 (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)

Anti -Angiopoietin 1 (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Tie-1 (human 1138aa; mouse 1134aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Murine and human Ang-1 are 498aa secreted angiogenic proteins. It is only 60% homologous with Ang-2. Ang-1 is the natural ligand for Tie-2 and is primarily expressed in developing endothelial cells.
Catalog #A2293
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:100,000 (50-100ng of control peptide/
well)
Western Blot: 1:1000-1:5000 (Expected size of Ang-1 is ~55kD)
Optimal dilutions to be determined by the researcher.
Recommended Control PeptideA2293-05: Angiopoietin 1, Control Peptide (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PurityNeat serum
ImmunogenSynthetic peptide corresponding to 20aa near the N-terminus of the mouse Ang-1 (KLH). Species Sequence Homology
SpecificityRecognizes mouse Ang-1 (~55kD). Detects ~70kD glycosylated, secreted protein in the conditioned media from cells expressing recombinant Ang-1. No significant sequence homology with Ang-2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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