Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Angiopoietin » Anti -Angiopoietin-like 3 (ANGPTL3, ANGPT5) (Biotin)

Anti -Angiopoietin-like 3 (ANGPTL3, ANGPT5) (Biotin)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins. Human ANGPTL3 contains an N-terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C-terminal fibrinogen-like domain that binds integrin alphavbeta3. In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N-terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C-terminal fragment that can induce angiogenesis. Full length and cleaved ANGPTL3 circulate in plasma. Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively.
Catalog #A2293-48D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml Detection limit for rhANGPLT3 is ~1ng/lane under non-reducing and reducing conditions. In this format, A2293-48D shows ~20% cross-reactivity with rmANGPTL3.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, TBS, 0.1% BSA. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with 1ml sterile 40-50% glycerol, TBS, 0.1% BSA. Labeled with Biotin.
PurityPurified by affinity chromatography.
ImmunogenSf21-derived rhANGPLT3 (aa 17-460).
SpecificityRecognizes human ANGPLT3. Species sequence homology: 76%, mouse; 83%, porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links