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Anti -Angiopoietin-like 3 (ANGPTL3, ANGPT5) (Biotin)
Anti -Angiopoietin-like 3 (ANGPTL3, ANGPT5) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins. Human ANGPTL3 contains an N-terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C-terminal fibrinogen-like domain that binds integrin alphavbeta3. In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N-terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C-terminal fragment that can induce angiogenesis. Full length and cleaved ANGPTL3 circulate in plasma. Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively. | | | Catalog # | A2293-48D | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-0.2ug/ml Detection limit for rhANGPLT3 is ~1ng/lane under non-reducing and reducing conditions. In this format, A2293-48D shows ~20% cross-reactivity with rmANGPTL3. | | Optimal dilutions should be determined by each laboratory for each application. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, TBS, 0.1% BSA. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~0.05mg/ml | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with 1ml sterile 40-50% glycerol, TBS, 0.1% BSA. Labeled with Biotin. | | Purity | Purified by affinity chromatography. | | Immunogen | Sf21-derived rhANGPLT3 (aa 17-460). | | Specificity | Recognizes human ANGPLT3. Species sequence homology: 76%, mouse; 83%, porcine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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