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You are here:Home » Antibodies » Abs to Angiopoietin » Anti -Angiopoietin-like 3 (ANGPTL3, ANGPT5)

Anti -Angiopoietin-like 3 (ANGPTL3, ANGPT5)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that belongs to the angiopoietin family. It is expressed in the liver and circulates as 70kD, 50kD, and 32kD species. These include full length ANGPTL3 as well as the proteolytically separated N- and C-terminal fragments which contain a coiled coil domain and fibrinogen-like domain, respectively. ANGPTL3 promotes an increase in circulating triglyceride levels by directly inhibiting lipoprotein lipase. Mature human ANGPTL3 shares 77% aa sequence identity with mouse ANGPTL3.
Catalog #A2293-48E
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml Using a colorimetric detection system, the detection limit for rhANGPTL3 is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
ELISA (direct): 0.5-1.0ug/ml Detects human ANGPTL3. The detection limit for rhANGPTL3 is ~6.5ng/well.
Optimal dilutions to be determined by the researcher.
HybridomaSf-21-derived myeloma cells with spleen B cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H146
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenSf-21-derived rhANGPTL3
SpecificityRecognizes human ANGPTL3. Species Crossreactivity: recombinant murine ANGPTL3-40%. Does not recognize recombinant human ANG1, ANG2 or ANG4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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