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You are here:Home » Molecular Biology » MB-Angiopoietin » ANGPTL5, Recombinant, Human, 6-His-Tag (Angiopoietin-related Protein 5, Angiopoietin-like Protein 5, UNQ5795/PRO19600)

ANGPTL5, Recombinant, Human, 6-His-Tag (Angiopoietin-related Protein 5, Angiopoietin-like
Protein 5, UNQ5795/PRO19600)

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Specifications

Angiopoietin-like 5 (ANGPTL5) is a secreted glycoprotein that is structurally related to the angiopoietins which contain an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (1-3). It shares 88% and 93% aa sequence identity with bovine and porcine ANGPTL5, and greater than 98% identity with chimpanzee, orangutan, and rhesus ANGPTL5. ANGPTL5 is expressed in adipose, bronchial, epididymal, and heart tissue (3, 4). Rare polymorphisms and loss of function mutations in human ANGPTL5 are associated with low circulating triglyceride levels and alterations in body mass index (4, 5). ANGPTL5, when used in combination with other growth factors such as SCF, Thrombopoietin, IGF-II, FGF acidic, Flt-3 Ligand, and IGFBP-2, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (6-8). The coiled-coil domain of ANGPTL proteins is critical for this effect. However, the molecular mechanism of ANGPTL proteins in stem cell activity remains unclear (6).
Catalog #A2293-49D
SourceRecombinant corresponding to aa1-388 of human Angiopoietin-like 5 (CFS), fused with 6-His tag at C-terminal, expressed in CHO cells.
Molecular Weight~42.3kD
Endotoxin Level 1EU/1ug (LAL)
Biological ActivityMeasured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of recombinant mouse SCF, recombinant mouse Thrombopoietin and recombinant mouse Flt-3 Ligand. The ED50 for this effect is typically 70-280ng/ml in the presence of a cross-linking mouse polyHistidine antibody.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~42.3kD
SourceCHO
Purity~90% (SDS-PAGE)
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, sodium chloride. Reconstitute with PBS to 100ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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