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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Angiostatin Kringles 1-3

Anti -Angiostatin Kringles 1-3

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis.
Catalog #A2294-30A
Recent studies have identified several proteolytic fragments or cryptic domains of proteins that act as inhibitors of angiogenesis. These include fragments of plasminogen such as Angiostatin protein (kringles 1-4) and kringles 1-5, C-terminal proteolytic fragment of Collagen XVIII (Endostatin protein), the NC10 domain of collagen 15 (Restin), the C-terminal hemopexin-like domain of MMP-2 (PEX), the N-terminal fragment of prolactin, and the N-terminally truncated platelet factor. Angiostatin protein, a proteolytic fragment of plasminogen, is comprised of the first four kringle regions. It prevents the growth of endothelial cells, and its systemic administration inhibits the growth of primary carcinomas in mice. Kringles 1-3 fragment has a greater inhibitory activity than the kringles 1-4 fragment. The protease-activated kringles 1-5 is the most potent plasminogen fragment with over 50-fold greater endothelial cell specific inhibitory activity. Its systemic administration inhibited the growth of fibrosarcoma and significantly reduced neovascularization.
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1-3 ug/ml. This antibody detects 10-50 ng recombinant protein.
ELISA: 0.5-2ug/ml using 100-200ng coated protein/well.
Optimal dilutions to be determined by the researcher.
Positive Control A2294-30B
Human K1-3 protein, formulated in SDS-PAGE sample buffer (reduced). This preparation is biologically inactive. It is not suitable for ELISA or other applications where native protein is required. It is supplied in 100 ul/vial. For WB, heat once and load 10 ul/lane and visualize with appropriate antibodies. This preparation is intended for qualitative purpose and not to serve as standard of known concentration.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No4C8
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified human Kringles 1-3 ~38kD, non-reducing, prepared from individuals that have been negative for HBsAg, HIV, and HCV. It is 95% pure as determined by SDS-PAGE. Purified Kringles 1-3 is free from plasmin or plasminogen.
SpecificityRecognizes human Angiostatin. Shows poor reactivity with mouse K1-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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